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Nucleic Acids Research, 1988, Vol. 16, No. 1 279-289
© 1988

Articles

Conformation of the synthetic DNA poly(amino2dA-dT) duplex in high-salt and aqueous alcohol solutions

Michaela Vorlicková, János Sági1, Anna Szabolcs1, Attila Szemzö1, László Ötvös1 and Jaroslav Kypr

Institute of Biophysics, Czechoslovak Academy of Sciences 612 65 Brno, Czechoslovakia Central Research Institute for Chemistry, Hungarian Academy of Sciences H-1525 Budapest, Hungary

Received November 4, 1987. Accepted December 9, 1987.

It has previously been demonstrated by other workers that the duplex of a synthetic DNA poly(amino2dA-dT) undergoes a salt-induced conformational isomerization. We show in the present work using circular dichroism that the same isomerization is induced in poly(amino2dA-dT) by various alcohols. The isomerization was originally identified as the B-to-Z and then B-to-A conformational transition of DNA but we demonstrate that the high-salt or alcohol conformation of poly (amino2dA-dT) is the non Z-DNA zig-zag double helix we have previously observed with poly(dA-dT) and called X-DNA. X-DNA is a cesium cation specific conformation of poly(dA-dT) while no similar cation specificity is observed with poly(amino2dA-dT). Thus it appears that the extra amino group attached to A and cesium cations make the same thing; they probably dehydrate the double helix minor groove and relieve its conformational variability. Poly(amino2dA-dT) is exceptionally stable in X-DNA and conditions inducing it are mild, which opens the door to assess its molecular structure.


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