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Nucleic Acids Research, 1988, Vol. 16, No. 1 39-50
© 1988

Articles

Root nodule specific gene regulation: analysis of the soybean nodulin N23 gene promoter in heterologous symbiotic systems

Jan-Elo Jørgensen, Jens Stougaard, Anne Marcker and Kjeld A. Marcker

Department of Molecular Biology and Plant Physiology, University of Aarhus C.F.Møllers Allé 130, DK-8000 Århus C, Denmark

Received October 22, 1987. Revised December 2, 1987. Accepted December 2, 1987.

The nodulin N23 gene promoter was analysed in transgenic plants using the chloramphenicol acetyltransferase (CAT) coding sequence as a reporter. A 5' flanking region of less than 1 Kb was sufficient for the organ-specific expression of a chimeric N23-CAT-3' lbc3 gene in root nodules formed on Lotus corniculatus and Trifolium repens after infection by their respective Rhizobium symbionts. Expression was regulated at the level of RNA in both species of transgenic plants. Promoter deletion analysis defined the 5' region required for high level expression and delimited two putative regulatory sequences involved in positive control of the N23 gene in L. corniculatus.


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Regulated Genes in Transgenic Plants
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