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Nucleic Acids Research, 1988, Vol. 16, No. 10 4341-4352
© 1988

Articles

Site-specific degradation of Streptomyces lividans DNA during electrophoresis in buffers contaminated with ferrous iron

Xiufen Zhou+, Zixin Deng+, John L. Firmin, David A. Hopwood and Tobias Kieser*

John Innes Institute and AFRC Institute of Plant Science Research Norwich, NR4 7UH, UK

* To whom correspondence should be addressed

Received February 25, 1988. Revised April 26, 1988. Accepted April 26, 1988.

Streptomyces lividans DNA contains a modification which makes it susceptible to double-strand cleavage during electrophoresis in buffers contaminated with ferrous iron (which may be present in some batches of EDTA). The cleavage of the DNA is site-specific and the average fragment size resulting from limit digestion of total S. lividans DNA is about 6kb. DNA from Streptomyces coelicolor A3(2) and several other Streptomyces strains, and from E. coli, is not cleaved under the same conditions. A S. lividans mutant has been isolated which lacks the DNA modification.

We suspect that many reports of "poor" preparations of S. lividans plasmids may be due to the above effect.

+ Present address: Department of Soils and Agricultural Chemistry, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China


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