Skip Navigation

This Article
Right arrow Print PDF (1813K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (36)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Angelov, D.
Right arrow Articles by Pashev, I.G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Angelov, D.
Right arrow Articles by Pashev, I.G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 10 4525-4538
© 1988

Articles

Protein—DNA crosslinking in reconstituted nucleohistone, nuclei and whole cells by picosecond UV laser irradiation

D. Angelov1, V.Yu. Stefanovsky, S.I. Dimitrov, V.R. Russanova, E. Keskinova1 and I.G. Pashev*

Institute of Molecular Biology Bulgarian Academy of Sciences 1113 Sofia, Bulgaria 1Institute of Solid State Physics, Bulgarian Academy of Sciences 1113 Sofia, Bulgaria

* To whom correspondence should be addressed

Received December 11, 1987. Revised April 14, 1988. Accepted April 14, 1988.

A picosecond UV laser was used to cross-link proteins to DNA in nuclei, whole cells and reconstituted nucleohistone. Irradiation of the nucleohistone resulted in crosslinking 15–20% of bound histones to DNA in a very short time (one or several picosecond pulses), the efficiency of crosslinking to single stranded DNA being higher than to double stranded DNA. All histones as well as high mobility group 1 proteins were identified in the covalently linked protein-DNA complexes upon irradiation of isolated nuclei and whole cells. A method is suggested for isolation of crosslinked material from cells and nuclei in amounts sufficient for further analysis. Experiments with reconstituted nucleohistones showed that upon irradiation at a constant dose the efficiency of crosslinking depended on the intensity of the light, thus suggesting a two-quantum process is involved in the reaction.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
T. Shapkina, S. Lappi, S. Franzen, and P. Wollenzien
Efficiency and pattern of UV pulse laser-induced RNA-RNA cross-linking in the ribosome
Nucleic Acids Res., March 3, 2004; 32(4): 1518 - 1526.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. Angelov, J. M. Vitolo, V. Mutskov, S. Dimitrov, and J. J. Hayes
Preferential interaction of the core histone tail domains with linker DNA
PNAS, May 24, 2001; (2001) 121171498.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
V. Mutskov, D. Gerber, D. Angelov, J. Ausio, J. Workman, and S. Dimitrov
Persistent Interactions of Core Histone Tails with Nucleosomal DNA following Acetylation and Transcription Factor Binding
Mol. Cell. Biol., November 1, 1998; 18(11): 6293 - 6304.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
V. J. Mutskov, V. R. Russanova, S. I. Dimitrov, and I. G. Pashev
Histones Associated with Non-nucleosomal Rat Ribosomal Genes Are Acetylated while Those Bound to Nucleosome-organized Gene Copies Are Not
J. Biol. Chem., May 17, 1996; 271(20): 11852 - 11857.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. Angelov, J. M. Vitolo, V. Mutskov, S. Dimitrov, and J. J. Hayes
Preferential interaction of the core histone tail domains with linker DNA
PNAS, June 5, 2001; 98(12): 6599 - 6604.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.