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Nucleic Acids Research, 1988, Vol. 16, No. 10 4621-4636
© 1988

Articles

Analysis of the complete nucleotide sequence of the Agrobacterium tumefaciens virB operon

David V. Thompson, Leo S. Melchers1,*, Ken B. Idler2, Rob A. Schilperoort1 and Paul J.J. Hooykaas1

Agrigenetics Corporation, Advanced Research Division 5649 East Buckeye Road, Madison, WI 53716, USA 1Biochemistry Laboratory, Department of Plant Molecular Biology, Leiden University Wassenaarseweg 64, 2333 AL Leiden, The Netherlands 2Abbott Laboratories, Abbott Park IL 60064, USA

* To whom correspondence should be addressed.

Received March 1, 1988. Revised April 21, 1988. Accepted April 21, 1988.

The complete nucleotide sequence of the virB locus, from the octopine Ti plasmid of Agrobacterium tumefaciens strain 15955, has been determined. In the large virB-operon (9600 nucleotides) we have identified eleven open reading frames, designated virB1 to virB11. From DNA sequence analysis it is proposed that nearly all VirB products, i.e. VirB1 to VirB9, are secreted or membrane associated proteins. Interestingly, both a membrane protein (VirB4) and a potential cytoplasmic protein (VirB11) contain the consensus amino acid sequence of ATP-binding proteins. In view of the conjugative T-DNA transfer model, the VirB proteins are suggested to act at the bacterial surface and there play an important role in directing T-DNA transfer to plant cells.


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