Skip Navigation

This Article
Right arrow Print PDF (2778K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (79)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Etessami, P.
Right arrow Articles by Stanley, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Etessami, P.
Right arrow Articles by Stanley, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 11 4811-4829
© 1988

Articles

Delimitation of essential genes of cassava latent virus DNA 2

Pantea Etessami, Rowena Callis, Susan Ellwood and John Stanley*

Department of Virus Research, John Innes Institute and AFRC Institute of Plant Science Research Colney Lane, Norwich, NR4 7UH, UK

*To whom correspondence should be addressed

Received March 28, 1988. Revised May 12, 1988. Accepted May 12, 1988.

Insertion and deletion mutagenesis of both extended open reading frames (ORFs) of cassava latent virus DNA 2 destroys infectivity. Infectivity is restored by coinoculating constructs that contain single mutations within different ORFs. Although frequent intermolecular recombination produces dominant parental-type virus, mutants can be retained within the virus population indicating that they are competent for replication and suggesting that rescue can occur by complementation of trans acting gene products. By cloning specific fragments into DNA 1 coat protein deletion vectors we have delimited the DNA 2 coding regions and provide substantive evidence that both are essential for virus infection. Although a DNA 2 component is unique to whitefly-transmitted geminiviruses, the results demonstrate that neither coding region is involved solely in insect transmission. The requirement for a bipartite genome for whitefly-transmitted geminiviruses is discussed.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
M. Hussain, S. Mansoor, S. Iram, A. N. Fatima, and Y. Zafar
The Nuclear Shuttle Protein of Tomato Leaf Curl New Delhi Virus Is a Pathogenicity Determinant
J. Virol., April 1, 2005; 79(7): 4434 - 4439.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. Hehnle, C. Wege, and H. Jeske
Interaction of DNA with the Movement Proteins of Geminiviruses Revisited
J. Virol., July 15, 2004; 78(14): 7698 - 7706.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
Y. Hong, J. Stanley, and R. van Wezel
Novel System for the Simultaneous Analysis of Geminivirus DNA Replication and Plant Interactions in Nicotiana benthamiana
J. Virol., December 15, 2003; 77(24): 13315 - 13322.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
J. S. Pita, V. N. Fondong, A. Sangaré, G. W. Otim-Nape, S. Ogwal, and C. M. Fauquet
Recombination, pseudorecombination and synergism of geminiviruses are determinant keys to the epidemic of severe cassava mosaic disease in Uganda
J. Gen. Virol., March 1, 2001; 82(3): 655 - 665.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.