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Nucleic Acids Research, 1988, Vol. 16, No. 11 5031-5038
© 1988


Articles

The relative importance of Escherichia coli exonuclease III and endonuclease IV for the hydrolysis of 3'-phosphoglycolate ends in polydeoxynucleotides

Brigitte Siwek, Suzanne Bricteux-Grégoire, Véronique Bailly and Walter G. Verly

Biochimie, Faculté des Sciences, Université de Li`ge Sart Tilman B6, 4000 Liège I, Belgium

Received March 28, 1988. Revised May 13, 1988. Accepted May 13, 1988.

In vitro, in the presence of Mg++, the 3'-phosphoglycolatase activity of endonuclease IV is about 4-times smaller than that of exonuclease III for the same AP endonuclease activity. It thus seems that endonuclease IV has only a minor role in the repair of strand breaks limited by 3'-phosphoglycolate ends in Escherichia coli even after the amount of enzyme has been increased by induction with O2 -generating agents.


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