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Nucleic Acids Research, 1988, Vol. 16, No. 11 5039-5053
© 1988

Articles

Circular dichroism studies of an oligo-{alpha}-thymidylate and of its interactions with complementary sequences

M. Durand, J. C. Maurizot*, N. T. Thuong and C. Helene1

Centre de Biophysique Moleculaire, 1A Avenue de la Recherche Scientifique 45071 Orleans, Cedex 2, France 1Laboratoire de Biophysique, INSERM U.201, Museum National d'Histoire Naturelle 61 Rue Buffon, 75005 Paris, France

*To whom correspondence should be addressed

Received March 28, 1988. Accepted May 6, 1988.

An octathymidylate synthesized with the {alpha} anomer of thymidine has been studied using circular dichroism. Its conformation has been compared to that of its analogue containing the naturally occurring ß anomer. In both compounds some degree of intramolecular stacking is present as indicated by the shapes of the circular dichroism spectra and their variations with temperature. As its ß- analogue the {alpha}-octathymidylate binds to its complementary sequences containing ß-nucleoeides. Only complexes with 1A:1T stoechiometry were observed. Binding to ribose- containing oligomers and polymers is much stronger than binding to deoxyribose-containing analogues. Circular dichroism spectra provided evidence for a difference between the geometry of the various complexes formed with an {alpha}-oligothymidylate and those formed with its ß-anomer-containing analogue.


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