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Nucleic Acids Research, 1988, Vol. 16, No. 11 5067-5073
© 1988

Articles

The oriC unwinding by dam methylation in Escherichia coli

Hiroshi Yamaki*, Eiichi Ohtsubo, Kazuo Nagai1 and Yoshimi Maeda

Institute of Applied Microbiology, University of Tokyo 1-1-1 Yayoi, Bunkyo-ku, 113 Tokyo, Japan 1Department of Agricultural Chemistry, University of Tokyo 1-1-1 Yayoi, Bunkyo-ku, 113 Tokyo, Japan

*To whom correspondence should be addressed

Received March 8, 1988. Revised May 12, 1988. Accepted May 12, 1988.

It has been shown that dam methylation is important in the regulation of initiation of DNA replication in E.coli. The question then arises as to whether dam methylation in the oriC region mediates any structural changes in DNA involved in the regulation of initiation of DNA replication. We demonstrate that the thermal melting temperature of the oriC region is lowered by adenine methylation at GATC sites. The regulation of initiation of DNA replication by dam methylation may be attributed to the ease of unwinding at GATC sites in oriC.


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T. Rein, T. Kobayashi, M. Malott, M. Leffak, and M. L. DePamphilis
DNA Methylation at Mammalian Replication Origins
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