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Nucleic Acids Research, 1988, Vol. 16, No. 12 5569-5585
© 1988

Articles

Developmentally regulated telomere addition in Tetrahymena thermophila

Elizabeth A. Spangler*, Thecla Ryan and Elizabeth H. Blackburn

Department of Molecular Biology, University of California Berkeley, CA 94720, USA

Received October 5, 1987. Revised January 19, 1988. Accepted January 19, 1988.

To investigate the developmentally programmed telomere addition that accompanies chromosome fragmentation during macronuclear differentiation in Tetrahymena thermophila. five representative telomeric regions from the macronucleus were cloned and characterized in detail. The sequences adjacent to the telomeric (C4A2:T2G4) repeats on these five macronuclear ends had no significant sequence homology or shared secondary structure. Two developmentally independent examples of one macronuclear telomere had a 5 base pair difference in the position of the junction between the telomeric repeats and the adjacent sequences. A telomere-adjacent sequence, in the form of a synthetic oligonucleotide, was unable to prime the addition of telomeric repeats in vitro. The implications of these results for the mechanisms underlying developmentally programmed chromosome fragmentation and telomere addition in Tetrahymena are discussed.

*Present address: Department of Genetics, University of Wisconsin, Madison, WI 53706, USA


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