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Nucleic Acids Research, 1988, Vol. 16, No. 13 5957-5972
© 1988

Articles

Purification and characterization of DNA methyltransferases fromNeisseria gonorrhoeae

Andrzej Piekarowicz1,2, Robert Yuan2 and Daniel C. Stein2

1Medical Biotechnology Center, University of Maryland Baltimore, MD 2Department of Microbiology, University of Maryland College Park, MD 20742, USA

Received March 9, 1988. Revised May 24, 1988. Accepted May 24, 1988.

Three DNA methyltransferases, M NgoAI, and M NgoBI and M NgoBII, free of any nuclease activities were isolated from Neisseria gonorrhoeae strains WR220 and MUG116 respectively. M NgoAI recognizes the sequence 5' GGCC 3' and methylates the first 5' cytosine on both strands. M NgoBI and M.NgoBII recognize 5' TCACC 3' and 5' GTAN5CTC 3' and 5' GTAN5CTC 3' respectively. M NgoBII methylates cytosine on only one strand to produce 5' GTAN5mCTC 3'.


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