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Nucleic Acids Research, 1988, Vol. 16, No. 13 6015-6022
© 1988

Articles

Pulsed-field electrophoresis indicates larger-than-expected sizes for mycoplasma genomes

Louise E. Pyle, Lynn N. Corcoran1, Benjamin G. Cocks, Andrew D. Bergemann, Jane C. Whitley and Lloyd R. Finch

Russell Grimwade School of Biochemistry, University of Melbourne Parkville, Victoria 3052, Australia 1Walter and Eliza Hall Institute Parkville, Victoria 3052, Australia

Received February 12, 1988. Revised June 1, 1988. Accepted June 1, 1988.

The sizes of large DNA fragments produced from genornes of members of the Mycoplasmataceae by digestion with restriction endonucleases having infrequent (1 to 3) cleavage sites within the genome were estimated from their mobility in contour-clamped homogeneous electric field (CHEF) agarose gel electrophoresis by comparison with yeast chromosomal DNA markers. The estimates of total genome size for 7 strains of 6 species ranged from approximately 900 kilo base pairs (kb) for Ureaplasma urealyticum 960T to 1330 kb for M. mycoides subsp. mycoides GC-1176. The values derived from this new method are considerably higher than those of approximately 500 Mdaltons or 750 kb previously reported for genome sizes in members of the Mycoplasmataceae.


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