Skip Navigation

This Article
Right arrow Print PDF (493K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Burgers, P. M.J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Burgers, P. M.J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 14 6297-6307
© 1988

Articles

Mammalian cyclin/PCNA (DNA polymerase {delta} auxiliary protein) stimulates processive DNA synthesis by yeast DNA polymerase III

Peter M.J. Burgers

Department of Biological Chemistry, Washington University School of Medicine St Louis, MO 63110, USA

Received February 24, 1988. Human cyclin/PCNA (proliferating cell nuclear antigen) is structurally, functionally, and immunologically homologous to the calf thymus auxiliary protein for DNA polymerase {delta}. This auxiliary protein has been investigated as a stimulatory factor for the nuclear DNA polymerases from S. cerevisiae. Calf cyclin/PCNA enhances by more than ten-fold the ability of DNA polymerase III to replicate templates with high template/primer ratios, e.g. poly(dA)·oligo(dT) (40:1). The degree of stimulation increases with the template/primer ratio. At a high template/primer ratio, i.e. low primer density, cyclin/PCNA greatly increases processive DNA synthesis by DNA polymerase III. At low template/primer ratios (e.g. poly(dA)·oligo(dT) (2.5:1), where addition of cyclin/PCNA only minimally increases the processivity of DNA polymerase III, a several-fold stimulation of total DNA synthesis is still observed. This indicates that cyclin/PCNA may also increase productive binding of DNA polymerase III to the template-primer and stabilize the template-primer-polymerase complex. The activity of yeast DNA polymerases I and II is not affected by addition of cyclin/PCNA. These results strengthen the hypothesis that yeast DNA polymerase III is functionally analogous to the mammalian DNA polymerase {delta}.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
J. Scorah, M.-Q. Dong, J. R. Yates III, M. Scott, D. Gillespie, and C. H. McGowan
A Conserved Proliferating Cell Nuclear Antigen-interacting Protein Sequence in Chk1 Is Required for Checkpoint Function
J. Biol. Chem., June 20, 2008; 283(25): 17250 - 17259.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
R. V. Skibbens, L. B. Corson, D. Koshland, and P. Hieter
Ctf7p is essential for sister chromatid cohesion and links mitotic chromosome structure to the DNA replication machinery
Genes & Dev., February 1, 1999; 13(3): 307 - 319.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
T. A. Ranalli, M. S. DeMott, and R. A. Bambara
Mechanism Underlying Replication Protein A Stimulation of DNA Ligase I
J. Biol. Chem., January 11, 2002; 277(3): 1719 - 1727.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.