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Nucleic Acids Research, 1988, Vol. 16, No. 14 6327-6338
© 1988

Articles

Identification and sequence of gene dicB: translation of the division inhibitor from an in-phase internal start

Kaymeuang Cam+, Samir Béjar, Dominique Gil and Jean-Pierre Bouché*

Centre de Recherches de Biochimie et de Génétique Cellulaires du CNRS 118 Route de Narbonne, 31062 Toulouse Cédex, France

*To whom correspondence should be addressed

Received February 24, 1988.

The dicAl mutation, located in the replication termination region of Escherichia coli at 34.9 min, confers a temperature-sensitive, division defective phenotype to its hosts. Previous analysis had suggested that dicA codes for a repressor of a nearby division inhibition gene dicB. We show now that gene dicB is part of a complex operon. Five open reading frames (ORFs 1 to 5) preceeded by a promoter sensitive to dicA repression are found within a 1500 bp segment, and are organized into two clusters separated by a long untranslated region. Evidence for expression of these ORFs was obtained from in vitro or in vivo translation of plasmid-coded genes. IPTG-dependent cell filamentation was obtained when either the entire or the C-terminal part of the fourth ORF was placed under control of the lac promoter. In both cases, a 7 KD protein corresponding to translation from an in-frame ATG of ORF4 (dicB) was made. We propose that this C-terminal protein is the division inhibitor synthesized in dicAl mutants.

+Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA


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