Skip Navigation

This Article
Right arrow Print PDF (927K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Sakmar, T. P.
Right arrow Articles by Khorana, H.G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sakmar, T. P.
Right arrow Articles by Khorana, H.G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 14 6361-6372
© 1988

Articles

Total synthesis and expression of a gene for the {alpha}-subunit of bovine rod outer segment guanine nucleotide-binding protein (transducin)

Thomas P. Sakmar and H.Gobind Khorana

Departments of Biology and Chemistry, Massachusetts Institute of Technology Cambridge, MA 02139, USA

Received February 29, 1988. To facilitate structure-function studies by site-specific mutagenesis, we have synthesized a gene for the {alpha}-subunit of the bovine rod outer segment (ROS) guanine nucleotide-binding protein (transducin). The gene codes for the native amino acid sequence and contains, by design, 38 unique restriction sites which are uniformly spaced. This enables mutagenesis in any part of the gene by restriction fragment replacement. The gene is 1076 base pairs in length. It was constructed from 44 synthetic oligonucleotides which were joined enzymatically in vitro into four fragments which were cloned. The synthetic transducin gene and cDNA encoding transducin were expressed to similar levels in monkey kidney cells (COS-1) using a vector in which transcription was under the control of the adenovirus major late promoter.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
C. G. Unson, A. M. Cypess, H. N. Kim, P. K. Goldsmith, C. J. L. Carruthers, R. B. Merrifield, and T. P. Sakmar
Characterization of Deletion and Truncation Mutants of the Rat Glucagon Receptor
J. Biol. Chem., November 17, 1995; 270(46): 27720 - 27727.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
M. Braiman, J. Bubis, T. Doi, H.-B. Chen, S.L. Flitsch, R.R. Franke, M.A. Gilles-Gonzalez, R.M. Graham, S.S. Karnik, H.G. Khorana, et al.
Studies on Light Transduction by Bacteriorhodopsin and Rhodopsin
Cold Spring Harb Symp Quant Biol, January 1, 1988; 53(0): 355 - 364.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
E. P. Marin, A. G. Krishna, and T. P. Sakmar
Rapid Activation of Transducin by Mutations Distant from the Nucleotide-binding Site. EVIDENCE FOR A MECHANISTIC MODEL OF RECEPTOR-CATALYZED NUCLEOTIDE EXCHANGE BY G PROTEINS
J. Biol. Chem., July 13, 2001; 276(29): 27400 - 27405.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. P. Marin, A. G. Krishna, V. Archambault, E. Simuni, W.-Y. Fu, and T. P. Sakmar
The Function of Interdomain Interactions in Controlling Nucleotide Exchange Rates in Transducin
J. Biol. Chem., June 22, 2001; 276(26): 23873 - 23880.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.