Skip Navigation

This Article
Right arrow Print PDF (1224K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Low, R. L.
Right arrow Articles by Couper, C. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Low, R. L.
Right arrow Articles by Couper, C. L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 14 6427-6445
© 1988

Articles

The preference of the mitochondrial endonuclease for a conserved sequence block in mitochondrial DNA is highly conserved during mammalian evolution

Robert L. Low*, Jenny M. Buzan and Christopher L. Couper

Departments of Pathology and Biological Chemistry, Washington University School of Medicine St Louis, MO 63110, USA

*To whom correspondence should be addressed

Received March 1, 1988. Endonuclease activity identified in crude preparations of rat and human-heart mitochondria has each been partially purified and characterized. Both the rat and human activities purify as a single enzyme that closely resembles the endonuclease of bovine-heart mitochondria (Cummings, O.W. et. al. (1987) J. Biol. Chem. 262:2005–2015). All three enzymes, for example elute similarly during gel filtration and DNA-cellulose chromatography, and exhibit similar enzymatic properties. Although the nucleotide sequences of the mtDNAs indicate that there has occurred an unusual degree of divergence in the displacement-loop region during mammalian evolution, the nucleotide specificities of the mt endonucleases appear highly conserved and show a striking preference for an evolutionarily-conserved sequence tract that is located up-stream from the heavy (H)-strand origin of DNA replication (OriH).


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
A. M. Davies, S. Hershman, G. J. Stabley, J. B. Hoek, J. Peterson, and A. Cahill
A Ca2+-induced mitochondrial permeability transition causes complete release of rat liver endonuclease G activity from its exclusive location within the mitochondrial intermembrane space. Identification of a novel endo-exonuclease activity residing within the mitochondrial matrix
Nucleic Acids Res., February 15, 2003; 31(4): 1364 - 1373.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
J Cote and A Ruiz-Carrillo
Primers for mitochondrial DNA replication generated by endonuclease G
Science, August 6, 1993; 261(5122): 765 - 769.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.