Skip Navigation

This Article
Right arrow Print PDF (1131K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Masai, H.
Right arrow Articles by Arai, K.-i.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Masai, H.
Right arrow Articles by Arai, K.-i.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 14 6493-6514
© 1988

Articles

RepA protein- and oriR-dependent initiation of R1 plasmid replication: identification of a rho-dependent transcription terminator required for cis-action of repA protein

Hisao Masai and Ken-ichi Arai

Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology 901 California Avenue, Palo Alto, CA 94304, USA

Received March 4, 1988. Initiation of R1 plasmid replication is dependent on cis-acting repA protein and the 188 base-pair (bp) sequence, oriR. RepA protein synthesized in vitro preferentially activates oriR in cis, regardless of the orientation and location of oriR on the template DNA. RepA protein is not reusable after it activates oriR in the cis-position. Cis-action of repA protein is also dependent on the presence of CIS, a 170 bp sequence, between repA and oriR. CIS contains a rho dependent transcription terminator of the repA transcript, deletion of which results in decrease in transformation efficiency and rapid loss of plasmid in the absence of selection. The significance of transcription termination events in replication was indicated by decreased replication activity in vivo caused by premature termination of the repA transcript between repA and CIS. A model which may account for the role of CIS in mediating the cis-action of the repA protein is presented.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Odegrip, D. Coomber, B. Eldridge, R. Hederer, P. A. Kuhlman, C. Ullman, K. FitzGerald, and D. McGregor
CIS display: In vitro selection of peptides from libraries of protein-DNA complexes
PNAS, March 2, 2004; 101(9): 2806 - 2810.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
J. Praszkier, S. Murthy, and A. J. Pittard
Effect of CIS on Activity in trans of the Replication Initiator Protein of an IncB Plasmid
J. Bacteriol., July 15, 2000; 182(14): 3972 - 3980.
[Abstract] [Full Text]

Home page
 J. Bacteriol.Home page
J. Praszkier and A. J. Pittard
Role of CIS in Replication of an IncB Plasmid
J. Bacteriol., May 1, 1999; 181(9): 2765 - 2772.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.