Skip Navigation

This Article
Right arrow Print PDF (1248K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Miyatake, S.
Right arrow Articles by Arai, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miyatake, S.
Right arrow Articles by Arai, N.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 14 6547-6566
© 1988

Articles

Activation of T cell-derived lymphokine genes in T cells and fibroblasts: effects of human T cell leukemia virus type I p40x protein and bovine papilloma virus encoded E2 protein

Shoichiro Miyatake, Motoharu Seiki1, Rene DeWaal Malefijt2, Toshio Heike, Jun-ichi Fujisawa1, Yutaka Takebe, Junji Nishida, Joseph Shlomai, Takashi Yokota, Mitsuaki Yoshida1, Ken-ichi Arai and Naoko Arai

Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology 901 California Avenue, Palo Alto, CA 94304-1104, USA 1Department of Viral Oncology, Cancer Institute Kami-Ikebukuro, Toshima-ku, Tokyo 170, Japan 2UNICET, Centre de Recherche 27 Chemin des Peupliers, BP11, 69572 Dardilly, France

Received March 9, 1988. The effects of p40x, a product of an human T cell leukemia virus type I, on the activation of lymphokine genes were examined. The mouse GM-CSF and IL-3 genes were activated by cotransfection with a pX containing plasmid both in Jurkat and CV1 cells. Mouse GM-CSF gene was also activated by phytohaemagglutinin A (PHA)/phorbol myristate acetate (PMA) or PMA/calcium ionophore A23187 [GenBank] stimulation. The 5'-flanking region of the mouse GM-CSF gene which is required for activation by pX or mitogen was mapped within 226 bp upstream from the transcription initiation site. Action of pX was not restricted to T cells. pX activated exogenously added GM-CSF, IL-2, IL-3 and IL-4 genes in fibroblasts. Activation of the GM-CSF gene in fibroblasts appears to require the same regulatory region as in T cells. Similar results were obtained using bovine papilloma virus encoded E2 protein. We propose that pX or E2 protein, both in T cells and fibroblasts, activates cellular component(s) in the signal transduction pathway which results in the activation of lymphokine genes in the absence of extracellular stimuli.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
M. G. Anderson, K. E. S. Scoggin, C. M. Simbulan-Rosenthal, and J. A. Steadman
Identification of Poly(ADP-Ribose) Polymerase as a Transcriptional Coactivator of the Human T-Cell Leukemia Virus Type 1 Tax Protein
J. Virol., March 1, 2000; 74(5): 2169 - 2177.
[Abstract] [Full Text]

Home page
 J. Virol.Home page
Y. Koya, T. Ohashi, H. Kato, S. Hanabuchi, T. Tsukahara, F. Takemura, K.-i. Etoh, M. Matsuoka, M. Fujii, and M. Kannagi
Establishment of a Seronegative Human T-Cell Leukemia Virus Type 1 (HTLV-1) Carrier State in Rats Inoculated with a Syngeneic HTLV-1-Immortalized T-Cell Line Preferentially Expressing Tax
J. Virol., August 1, 1999; 73(8): 6436 - 6443.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
I. Rivera, E. W. Harhaj, and S.-C. Sun
Involvement of NF-AT in Type I Human T-cell Leukemia Virus Tax-mediated Fas Ligand Promoter Transactivation
J. Biol. Chem., August 28, 1998; 273(35): 22382 - 22388.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J.H. Richardson, P. Hollsberg, A. Windhagen, L.A. Child, D.A. Hafler, and A.M.L. Lever
Variable Immortalizing Potential and Frequent Virus Latency in Blood-Derived T-Cell Clones Infected With Human T-Cell Leukemia Virus Type I
Blood, May 1, 1997; 89(9): 3303 - 3314.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Kochetkova and M. F. Shannon
DNA Triplex Formation Selectively Inhibits Granulocyte-Macrophage Colony-stimulating Factor Gene Expression in Human T Cells
J. Biol. Chem., June 14, 1996; 271(24): 14438 - 14444.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
P. Hollsberg and D. A. Hafler
Pathogenesis of Diseases Induced by Human Lymphotropic Virus Type I Infection
N. Engl. J. Med., April 22, 1993; 328(16): 1173 - 1182.
[Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.