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Nucleic Acids Research, 1988, Vol. 16, No. 14 6607-6616
© 1988

Articles

The helical repeat of underwound DNA in solution

S.M.H. Richardson, T.C. Boles* and N.R. Cozzarelli

Department of Molecular Biology, University of California Berkeley, CA 94720, USA

Received April 7, 1988.

Closed circular DNA was relaxed with a topoisomerase in the presence of varying concentrations of the intercalating dye, ethidium bromide, to create underwound, planar DNA rings. We directly determined the helical repeat of these DNA molecules by the Gaussian center method and found that it varied as a simple predicted function of the degree of underwinding and the helical repeat of relaxed, dye-free DNA. We discuss these results in light of a recent mathematical treatment of DNA structure which predicts that the helical repeat of supercoiled DNA molecules in solution obeys the same function.

*Present address: British Biotechnology Ltd., Brook House, Watlington Road, Cowley, Oxford OX4 5LY, UK


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