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Nucleic Acids Research, 1988, Vol. 16, No. 14 6767-6778
© 1988

Articles

CpG islands and HTF islands in the HLA class I region: investigation of the methylation status of class I genes leads to precise physical mapping of the HLA-B and -C genes

P. Pontarotti, G. Chimini1, C. Nguyen2, J. Boretto and B.R. Jordan

CIML INSERM-CNRS Luminy, Case 906, 13288 Marseille Cedex 9, France 1Istituto Nazionale per Ia ricerca sul Cancro Viale Benedetto XV n° 10 Genova, Italy 2CIML INSERM-CNRS INSERM U 242, Hôpital des enfants de Ia Timone, 13005 Marseille, France

Received April 29, 1988. Revised June 14, 1988. Accepted June 14, 1988.

We have investigated the accessibility of the 5' CpG rich sequences (CpG islands) present in the 5' region of most if not all HLA class I genes to methylation sensitive rare cutter enzymes. We show that for HLA-A, -B, -c genes and a few other (but not all) class I sequences these CpG islands are unmethylated and therefore constitute HTF islands (cpG rich, unmethylated regions of DNA, usually associated with expressed genes). We then map precisely the HTF islands of the HLA-B and HLA-C genes and determine that they are separated by 130 Kb (in agreement with genetic data) and that these two genes are in the same transcriptional orientation on the chromosome.


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