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Nucleic Acids Research, 1988, Vol. 16, No. 15 7383-7395
© 1988

Articles

Selection of cryptic 5' splice sites by group II intron RNAs in vitro

Manfred W. Müller, Rudolf J. Schweyen1 and Carlo Schmelzer

Institut für Genetik und Mikrobiologie, Universität M7uuml;nchen Maria-Ward-Str. 1A, 8000 Müuchen 19, FRG 1Institute für Genetik und Mikrobiologie, Universität Wein Althanstr. 14, 1090 Wein, Austria

Received May 16, 1988. Revised July 4, 1988. Accepted July 4, 1988.

Recognition of 5' splice points by group I and group II self-splicing introns involves the interaction of exon sequences - directly preceding the 5' splice site - with intronic sequence elements. We show here that the exon binding sequences (EBS) of group II intron al5c can accept various substitutes of the authentic intron binding sites (IBS) provided in cis or in trans. The efficiency of cleavages at these cryptic 5' splice sites was enhanced by deletion of the authentic IBS2 element. All cryptic 5' cleavage sites studied here were preceded by an IBS1 like sequence; indicating that the IBS1/EBS1 pairing alone is sufficient for proper 5' splice site selection by the intronic EBS clement. The results are discussed in terms of minimal requirements for 5' cleavages and position effects of IBS sites relative to the intron.


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W D Downs and T R Cech
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Genes & Dev., May 15, 1994; 8(10): 1198 - 1211.
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