Nucleic Acids Research, 1988, Vol. 16, No. 15 7583-7600
© 1988
Articles |
ZAP: a bacteriophage expression vector with in vivo excision properties
Stratagene Cloning Systems 11099 North Torrey Pines Road, La Jolla, CA 92037, USA
*To whom correspondence should be addressed
Received February 16, 1988. Revised June 29, 1988. Accepted June 29, 1988.
A lambda Insertion type cDNA cloning vector, Lambda ZAP, has been constructed. In E. coli a phagemid, pBluescript SK(–), contained within the vector, can be excised by 11 or M13 helper phage. The excision process elimlnates the need to subclone DNA inserts from the lambda phage into a plasmid by restriction digestion and ligation. This is possible because Lambda ZAP incorporates the signals for both initiation and termination of DNA synthesis from the 11 bacteriophage origin of replication (1). Six of 21 restriction sites In the excised pBluescript SK polylinker, contained within the NH2-portion of the lacZ gene, are unique in lambda ZAP. Coding sequences inserted into these restriction sites, in the appropriate reading frame, can be expressed from the lacZ promoter as fusion proteins. The features of this vector significantly increase the rate at which clones can be isolated and analyzed.
The lambda ZAP vector was tested by the preparation of a chicken liver cDNA Hbrary and the isolatlon of actin clones by screening with oligonucleotide probes. Putative actin clones were excised from the lambda vector and Identified by DNA sequencing. The ability of lambda ZAP to serve as a vector for the construction of cDNA expression libraries was determined by detecting fusion proteins from clones containing glucocerbrosidase cDNA's using rabbit IgG anti-glucocerbrosidase antibodies.
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E. Masai, K. Momose, H. Hara, S. Nishikawa, Y. Katayama, and M. Fukuda Genetic and Biochemical Characterization of 4-Carboxy-2-Hydroxymuconate-6-Semialdehyde Dehydrogenase and Its Role in the Protocatechuate 4,5-Cleavage Pathway in Sphingomonas paucimobilis SYK-6 J. Bacteriol., December 1, 2000; 182(23): 6651 - 6658. [Abstract] [Full Text]
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J. Guzzo and M. S. Dubow A Novel Selenite- and Tellurite-Inducible Gene in Escherichia coli Appl. Envir. Microbiol., November 1, 2000; 66(11): 4972 - 4978. [Abstract] [Full Text]
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I. Tatsuno, H. Kimura, A. Okutani, K. Kanamaru, H. Abe, S. Nagai, K. Makino, H. Shinagawa, M. Yoshida, K. Sato, et al. Isolation and Characterization of Mini-Tn5Km2 Insertion Mutants of Enterohemorrhagic Escherichia coli O157:H7 Deficient in Adherence to Caco-2 Cells Infect. Immun., October 1, 2000; 68(10): 5943 - 5952. [Abstract] [Full Text] [PDF]
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M. E. Ehsani, T. W. Abraha, C. Netherland-Snell, N. Mueller, M. M. Taylor, and B. Holwerda Generation of Mutant Murine Cytomegalovirus Strains from Overlapping Cosmid and Plasmid Clones J. Virol., October 1, 2000; 74(19): 8972 - 8979. [Abstract] [Full Text]
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 J. S. Kruszewska, M. Saloheimo, A. Migdalski, P. Orlean, M. Penttila, and G. Palamarczyk Dolichol phosphate mannose synthase from the filamentous fungus Trichoderma reesei belongs to the human and Schizosaccharomyces pombe class of the enzyme Glycobiology, October 1, 2000; 10(10): 983 - 991. [Abstract] [Full Text] [PDF]
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B. Kather, K. Stingl, M. E. van der Rest, K. Altendorf, and D. Molenaar Another Unusual Type of Citric Acid Cycle Enzyme in Helicobacter pylori: the Malate:Quinone Oxidoreductase J. Bacteriol., June 1, 2000; 182(11): 3204 - 3209. [Abstract] [Full Text]
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 N. Harris, J. M. Foster, A. Kumar, H. V. Davies, C. Gebhardt, and J. L. Wray Two cDNAs representing alleles of the nitrate reductase gene of potato (Solanum tuberosum L. cv. Desiree): sequence analysis, genomic organization and expression J. Exp. Bot., June 1, 2000; 51(347): 1017 - 1026. [Abstract] [Full Text] [PDF]
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B. N. Fry, S. Feng, Y.-Y. Chen, D. G. Newell, P. J. Coloe, and V. Korolik The galE Gene of Campylobacter jejuni Is Involved in Lipopolysaccharide Synthesis and Virulence Infect. Immun., May 1, 2000; 68(5): 2594 - 2601. [Abstract] [Full Text] [PDF]
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R. A. Kingsley, K. van Amsterdam, N. Kramer, and A. J. Baumler The shdA Gene Is Restricted to Serotypes of Salmonella enterica Subspecies I and Contributes to Efficient and Prolonged Fecal Shedding Infect. Immun., May 1, 2000; 68(5): 2720 - 2727. [Abstract] [Full Text] [PDF]
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R. Mitra, P. Figueroa, A. K. Mukhopadhyay, T. Shimada, Y. Takeda, D. E. Berg, and G. B. Nair Cell Vacuolation, a Manifestation of the El Tor Hemolysin of Vibrio cholerae Infect. Immun., April 1, 2000; 68(4): 1928 - 1933. [Abstract] [Full Text] [PDF]
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A. F. O'Donnell, S. Tiong, D. Nash, and D. V. Clark The Drosophila melanogaster ade5 Gene Encodes a Bifunctional Enzyme for Two Steps in the de novo Purine Synthesis Pathway Genetics, March 1, 2000; 154(3): 1239 - 1253. [Abstract] [Full Text]
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L. Gremer, S. Kellner, H. Dobbek, R. Huber, and O. Meyer Binding of Flavin Adenine Dinucleotide to Molybdenum-containing Carbon Monoxide Dehydrogenase from Oligotropha carboxidovorans. STRUCTURAL AND FUNCTIONAL ANALYSIS OF A CARBON MONOXIDE DEHYDROGENASE SPECIES IN WHICH THE NATIVE FLAVOPROTEIN HAS BEEN REPLACED BY ITS RECOMBINANT COUNTERPART PRODUCED IN ESCHERICHIA COLI J. Biol. Chem., January 21, 2000; 275(3): 1864 - 1872. [Abstract] [Full Text] [PDF]
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R. M. Tsolis, S. M. Townsend, E. A. Miao, S. I. Miller, T. A. Ficht, L. G. Adams, and A. J. Baumler Identification of a Putative Salmonella enterica Serotype Typhimurium Host Range Factor with Homology to IpaH and YopM by Signature-Tagged Mutagenesis Infect. Immun., December 1, 1999; 67(12): 6385 - 6393. [Abstract] [Full Text] [PDF]
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Z. Jiang and G. M. Shackleford Mouse Mammary Tumor Virus Carrying a Bacterial supF Gene Has Wild-Type Pathogenicity and Enables Rapid Isolation of Proviral Integration Sites J. Virol., December 1, 1999; 73(12): 9810 - 9815. [Abstract] [Full Text]
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R. L. Meighan-Mantha, D. K. W. Hsu, Y. Guo, S. A. N. Brown, S.-L. Y. Feng, K. A. Peifley, G. F. Alberts, N. G. Copeland, D. J. Gilbert, N. A. Jenkins, et al. The Mitogen-inducible Fn14 Gene Encodes a Type I Transmembrane Protein that Modulates Fibroblast Adhesion and Migration J. Biol. Chem., November 12, 1999; 274(46): 33166 - 33176. [Abstract] [Full Text] [PDF]
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T. L. Norris and A. J. Baumler Phase variation of the lpf operon is a mechanism to evade cross-immunity between Salmonella serotypes PNAS, November 9, 1999; 96(23): 13393 - 13398. [Abstract] [Full Text] [PDF]
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H. Ikadai, X. Xuan, I. Igarashi, S. Tanaka, T. Kanemaru, H. Nagasawa, K. Fujisaki, N. Suzuki, and T. Mikami Cloning and Expression of a 48-Kilodalton Babesia caballi Merozoite Rhoptry Protein and Potential Use of the Recombinant Antigen in an Enzyme-Linked Immunosorbent Assay J. Clin. Microbiol., November 1, 1999; 37(11): 3475 - 3480. [Abstract] [Full Text]
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P. M. Mathisen, J. M. Johnson, J. A. Kawczak, and V. K. Tuohy Visinin-like Protein (VILIP) Is a Neuron-specific Calcium-dependent Double-stranded RNA-binding Protein J. Biol. Chem., October 29, 1999; 274(44): 31571 - 31576. [Abstract] [Full Text] [PDF]
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I. Noll, S. Müller, and A. Klein Transcriptional Regulation of Genes Encoding the Selenium-Free [NiFe]-Hydrogenases in the Archaeon Methanococcus voltae Involves Positive and Negative Control Elements Genetics, August 1, 1999; 152(4): 1335 - 1341. [Abstract] [Full Text]
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A. Kraft, C. Lutz, A. Lingenhel, P. Gröbner, and W. Piendl Control of Ribosomal Protein L1 Synthesis in Mesophilic and Thermophilic Archaea Genetics, August 1, 1999; 152(4): 1363 - 1372. [Abstract] [Full Text]
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H. Merzendorfer, M. Huss, R. Schmid, W. R. Harvey, and H. Wieczorek A Novel Insect V-ATPase Subunit M9.7 Is Glycosylated Extensively J. Biol. Chem., June 11, 1999; 274(24): 17372 - 17378. [Abstract] [Full Text] [PDF]
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R. P. de Vries, H. C. van den Broeck, E. Dekkers, P. Manzanares, L. H. de Graaff, and J. Visser Differential Expression of Three alpha -Galactosidase Genes and a Single beta -Galactosidase Gene from Aspergillus niger Appl. Envir. Microbiol., June 1, 1999; 65(6): 2453 - 2460. [Abstract] [Full Text]
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P. M. O'Brien, R. Aitken, B. W. O'Neil, and M. S. Campo Generation of native bovine mAbs by phage display PNAS, January 19, 1999; 96(2): 640 - 645. [Abstract] [Full Text] [PDF]
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E. Masai, S. Shinohara, H. Hara, S. Nishikawa, Y. Katayama, and M. Fukuda Genetic and Biochemical Characterization of a 2-Pyrone-4,6-Dicarboxylic Acid Hydrolase Involved in the Protocatechuate 4,5-Cleavage Pathway of Sphingomonas paucimobilis SYK-6 J. Bacteriol., January 1, 1999; 181(1): 55 - 62. [Abstract] [Full Text]
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C. Pasternak, K. Haberzettl, and G. Klug Thioredoxin Is Involved in Oxygen-Regulated Formation of the Photosynthetic Apparatus of Rhodobacter sphaeroides J. Bacteriol., January 1, 1999; 181(1): 100 - 106. [Abstract] [Full Text]
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J. Hani, B. Schelbert, A. Bernhardt, H. Domdey, G. Fischer, K. Wiebauer, and J.-U. Rahfeld Mutations in a Peptidylprolyl-cis/trans-isomerase Gene Lead to a Defect in 3'-End Formation of a Pre-mRNA in Saccharomyces cerevisiae J. Biol. Chem., January 1, 1999; 274(1): 108 - 116. [Abstract] [Full Text] [PDF]
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M. Yamabhai, N. G. Hoffman, N. L. Hardison, P. S. McPherson, L. Castagnoli, G. Cesareni, and B. K. Kay Intersectin, a Novel Adaptor Protein with Two Eps15 Homology and Five Src Homology 3 Domains J. Biol. Chem., November 20, 1998; 273(47): 31401 - 31407. [Abstract] [Full Text] [PDF]
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O. Tureci, U. Sahin, E. Vollmar, S. Siemer, E. Gottert, G. Seitz, A.-K. Parkkila, G. N. Shah, J. H. Grubb, M. Pfreundschuh, et al. Human carbonic anhydrase XII: cDNA cloning, expression, and chromosomal localization of a carbonic anhydrase gene that is overexpressed in some renal cell cancers PNAS, June 23, 1998; 95(13): 7608 - 7613. [Abstract] [Full Text] [PDF]
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R. Angelo and C. S. Rubin Molecular Characterization of an Anchor Protein (AKAPCE) That Binds the RI Subunit (RCE) of Type I Protein Kinase A from Caenorhabditis elegans J. Biol. Chem., June 5, 1998; 273(23): 14633 - 14643. [Abstract] [Full Text] [PDF]
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A. W. M. van der Velden, A. J. Baumler, R. M. Tsolis, and F. Heffron Multiple Fimbrial Adhesins Are Required for Full Virulence of Salmonella typhimurium in Mice Infect. Immun., June 1, 1998; 66(6): 2803 - 2808. [Abstract] [Full Text] [PDF]
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 B. M. Sandmaier, R. Storb, K. L. Bennett, F. R. Appelbaum, and E. B. Santos Epitope Specificity of CD44 for Monoclonal Antibody-Dependent Facilitation of Marrow Engraftment in a Canine Model Blood, May 1, 1998; 91(9): 3494 - 3502. [Abstract] [Full Text] [PDF]
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O. Tureci, U. Sahin, C. Zwick, M. Koslowski, G. Seitz, and M. Pfreundschuh Identification of a meiosis-specific protein as a member of the class of cancer/testis antigens PNAS, April 28, 1998; 95(9): 5211 - 5216. [Abstract] [Full Text] [PDF]
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 B. Kohl, C. A Wagner, B. Huelseweh, A. E Busch, and A. Werner The Na+-phosphate cotransport system (NaPi-II) with a cleaved protein backbone: implications on function and membrane insertion J. Physiol., April 15, 1998; 508(2): 341 - 350. [Abstract] [Full Text] [PDF]
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R. Ross, X.-L. Ross, J. Schwing, T. Langin, and A. B. Reske-Kunz The Actin-Bundling Protein Fascin Is Involved in the Formation of Dendritic Processes in Maturing Epidermal Langerhans Cells J. Immunol., April 15, 1998; 160(8): 3776 - 3782. [Abstract] [Full Text] [PDF]
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