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Nucleic Acids Research, 1988, Vol. 16, No. 16 7773-7782
© 1988

Articles

KRAS codon 12 mutations occur very frequently in pancreatic adenocarcinomas

Vincent T.H.B.M. Smit, Angelina J.M. Boot1, Alida M.M. Smits1, Gert Jan Fleuren, Cees J. Cornelisse and Johannes L. Bos1,*

Department of Pathology State University of Leiden PO Box 9305, 2300 RA Leiden, The Netherlands 1Laboratory for Molecular Carcinogenesis, State University of Leiden PO Box 9305, 2300 RA Leiden, The Netherlands

*To whom correspondence should be addressed

Received July 13, 1988. Accepted July 26, 1988.

DNAs from human pancreatic adenocarcinomas were analyzed for the presence of mutations in codons 12, 13 and 61 of the NRAS, KRAS and HRAS gene. Formalin-fixed and paraffin-embedded tissue was used directly in an in vitro amplification reaction to expand the relevant RAS sequences. The mutations were detected by selective hybridization using mutation-specific synthetic oligonucleotides. In 28 of the 30 patients we found a mutation in codon 12 of the KRAS gene. This result confirms the findings of Almoguera et al. [Cell 53 (1988) 549–554] that KRAS mutations occur frequently in adenocarcinomas of the exocrine pancreas. The mutations are predominantly G-T transversions, in contrast to the KRAS mutations in colon tumors which are mainly G-A transitions. Furthermore, in a portion of the tumors the mutation appears to be homozygous.


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