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Nucleic Acids Research, 1988, Vol. 16, No. 16 8011-8028
© 1988

Articles

The organization and transcription of the galactose gene cluster of Kluyveromyces lactis

Thomas D. Webster+ and Robert C. Dickson*

Department of Biochemisny, University of Kentucky Medical Center Lexington, KY 40536–0084, USA

+Present address: Institute for Molecular Genetics, T813, Baylor College of Medicine, Texas Medical Center, Houston, TX 77030, USA

*To whom correspondence should be addressed

Received March 2, 1988. Revised July 18, 1988. Accepted July 18, 1988.

The yeast Kluyveromvces lactis grows on galactose by inducing the Leloir pathway enzymes-kinase, epimerase, and transferase. To investigate the molecular mechanism for reaulating expression of this metabolic pathway we isolated GAL1 GAL7, GAL10, which code for kinase, transferase, and epimerase, respectively and characterized their size, organization, and transcriptional regulation. Our results indicate that induction of the Leloir pathway in K. lactis occurs at the level of transcription and that the organization and regulation of the GAL gene cluster in K. lactis is closely related to the homologous gene cluster in Saccharomyces cerevisiae. Likewise, the Upstream Activator Sequences that regulate induction of the GAL aenes are similar in base sequence, number and relative location in the two yeasts.


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