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Nucleic Acids Research, 1988, Vol. 16, No. 16 8057-8076
© 1988

Articles

Multiple elements are required for expression of an intermediate filament gene

Christina M. Sax, Francis X. Farrell1, Janet A. Tobian2 and Zendra E. Zehner1,*

Department of Human Genetics MD 20205, USA 1Department of Biochemistry and Molecular Biophysics, The Massey Cancer Center, Medical College of Virginia, Virginia Commonwealth University Richmond, VA 23298, USA 2Human Genetics Branch, NTCHD, The National Institutes of Health Bethesda, MD 20205, USA

*To whom correspondence should be addressed

Received February 22, 1988. Revised July 18, 1988. Accepted July 18, 1988.

The expression of vimentin is unique within the intermediate filament multigene family. It is the only member which deviates from its usual tis-sue-specific expression pattern and whose 5'-flanking region contains multiple GC boxes, the binding site for Spl. The activity of vimentin 5'-end: CAT fusions has been compared in cells where vimentin is highly expressed (mouse L cells) or not expressed at all (MH1C1). In addition, CAT activity has been examined by microinjection into Xenopus oocytes. Both in vivo expression and in vivo binding studies implicate Sp1 as a general regulatory factor in vimentin gene expression. Increased expression of 5'-end: cat fusions in mouse L cells suggests that a fibroblast-specific enhancer element resides in the region –321 to –160. Low transcriptional activity in MH1C1 cells may be due to either the lack of this positive transcription factor(s) or the presence of a repressor element. Here, we demonstrate that the unique and complex pattern of vimentin gene expression Is controlled by multiple cis-acting elements.


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