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Nucleic Acids Research, 1988, Vol. 16, No. 16 8113-8128
© 1988

Articles

Reintedness of archaebacterial RNA polymerase core subunits to their eubacterial and eultaryotic equivalents

Bruno Berghöfer, Lothar Kröckel, Chrisliane Körtner+, Mathias Truss§, Jürgen Schallenberg and Albrecht Klein*

Molecular Genetics, Department of Bio1og Philipps-University Karl-von-Frisch-Strasse, D-3550 Marburg, FRG

+Present addresses: Institut fur Mikrobiologie, Theodor-Stern-Kai, D-6000 Frankfurt-M

§Present addresses: Institut fur Motekularbiologie und Tumorforschung, Philipps-University, Ernil-Mannkopff-Str.1, D-3550 Maiburg, FRG

*To whom correspondence should be addressed

Received June 17, 1988. Accepted July 26, 1988.

The sequence of the genes encoding the four largest sub units of the RNA polymerase of the archaebacterium Methanobacterium thermoautotrophicum was determined and putative trans lation signals were identified. The genes are more strongly homologous to eukaryotic than to eubacterial RNA polymerase genes. Analysis of the polypeptide sequences revealed co linearity of two pairs of adjacent archaebacterial genes en coding the B'' and B' or A and C genes, respectively, with two eubacterial and two eukaryotic genes each encoding the two largest RNA polymerase subunits. This difference in sequence organization is discussed in terms of gene fusion in the course of evolution. The degree of conservation is much higher between the archaebacterial and the eukaryotic polypeptides than between the archaebacterial and the eubacterial enzyme. Putative func tional domains were identified in two of the subunits of the archaebacterial enzyme.


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