Nucleic Acids Research, 1988, Vol. 16, No. 17 8467-8486
© 1988
Articles |
Multiple copies of a G-rich element upstream of a cAMP-inducible Dictyostelium gene are necessary but not sufficient for efficient gene expression
Imperial Cancer Research Fund, Clare Hall Laboratories Blanche Lane, South Mimms, Herts. EN6 3LD, UK
*To whom correspondence should be addressed
Received April 29, 1988. Revised August 2, 1988. Accepted August 2, 1988.
The cysteine proteinase 1 (CP1) and cysteine proteinase 2 (CP2) genes of Dictyostelium discoideum encode co-ordinately expressed mRNA sequences which are inducible by extracellular cAMP. There are short, G-rich sequence elements upstream of both genes and we have previously shown that deletion of these elements from the CP2 gene abolishes cAMP-inducibility. We show here that the G-rich element from the CP1 gene is functionally homologous to that in the CP2 gene by reconstituting cAMP-inducibility in a deletion mutant of the CP2 gene using CP1-derived sequences. Both the CPl and CP2 genes contain multiple G-rich elements. We show that efficient induction requires at least two copies of the CPl element and that their relative orientation is unimportant. Two copies of an inverted relative orientation are, however, inactive when moved upstream of their normal position and are incapable of conferring cAMP-inducibility on a heterologous gene. These observations suggest that these sequences are either essential promoter elements, not themselves interacting with the inducer, or that their interaction with a separate class of control sequences is necessary for inducible expression.
+Present address: Ludwig Institute, 91 Riding House Street, London, WIP 8T, UK
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  N. V. Zhukovskaya, M. Fukuzawa, Y. Yamada, T. Araki, and J. G. Williams The Dictyostelium bZIP transcription factor DimB regulates prestalk-specific gene expression Development, February 1, 2006; 133(3): 439 - 448. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Fukuzawa and J. Williams Analysis of the promoter of the cudA gene reveals novel mechanisms of Dictyostelium cell type differentiation Development, January 6, 2000; 127(12): 2705 - 2713. [Abstract] [PDF]
|
|
|
|
|
|
R Gollop and A. Kimmel Control of cell-type specific gene expression in Dictyostelium by the general transcription factor GBF Development, January 9, 1997; 124(17): 3395 - 3405. [Abstract] [PDF]
|
|
|
|
 |
|
 R A Firtel Integration of signaling information in controlling cell-fate decisions in Dictyostelium. Genes & Dev., June 15, 1995; 9(12): 1427 - 1444. [PDF]
|
|
|
|
|
|
G R Schnitzler, W H Fischer, and R A Firtel Cloning and characterization of the G-box binding factor, an essential component of the developmental switch between early and late development in Dictyostelium. Genes & Dev., February 15, 1994; 8(4): 502 - 514. [Abstract] [PDF]
|
|
|
|
|
|
A. Early, M. Gaskell, D Traynor, and J. Williams Two distinct populations of prestalk cells within the tip of the migratory Dictyostelium slug with differing fates at culmination Development, January 6, 1993; 118(2): 353 - 362. [Abstract] [PDF]
|
|
|
|
 |
|
 R. Jin, K. Breslauer, R. Jones, and B. Gaffney Tetraplex formation of a guanine-containing nonameric DNA fragment Science, October 26, 1990; 250(4980): 543 - 546. [Abstract] [PDF]
|
|
|
|
|
|
L Haberstroh and R A Firtel A spatial gradient of expression of a cAMP-regulated prespore cell-type-specific gene in Dictyostelium. Genes & Dev., April 1, 1990; 4(4): 596 - 612. [Abstract] [PDF]
|
|
|
|
|
|
A L Hjorth, C Pears, J G Williams, and R A Firtel A developmentally regulated trans-acting factor recognizes dissimilar G/C-rich elements controlling a class of cAMP-inducible Dictyostelium genes. Genes & Dev., March 1, 1990; 4(3): 419 - 432. [Abstract] [PDF]
|
|
|
|
|
|
A L Hjorth, N C Khanna, and R A Firtel A trans-acting factor required for cAMP-induced gene expression in Dictyostelium is regulated developmentally and induced by cAMP. Genes & Dev., June 1, 1989; 3(6): 747 - 759. [Abstract] [PDF]
|
|