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Nucleic Acids Research, 1988, Vol. 16, No. 17 8541-8554
© 1988

Articles

The human liver glutathione S-transferase gene superfamily: expression and chromosome mapping of an Hb subunit cDNA

Jeff L. DeJong1,2, Chi-Ming Chang2, Jacqueline Whang-Peng3, Turid Knutsen3 and Chen-Pei D. Tu1,2,*

1Department of Molecular and Cell Biology, Pennsylvania State University, University Park PA 16802, USA 2Institute of Molecular Biology Academia Sinica, Taipei 11529, China 3Medicine Branch NCI, NIH, Bethesda, MD 20892, USA

*To whom correspondence should be addressed

Received April 7, 1988. Revised July 25, 1988. Accepted July 25, 1988.

We have isolated from a {lambda}gtl0 cDNA library a clone {lambda}GTH4 which encodes a human liver glutathione S-transferase Hb subunit, designated as subunit 4. Expression of this cDNA in E. coli and subsequent purification and immunoblotting analysis provided a definitive assignment of a structure and function relationship. RNA blot hybridization with human liver poly(A) RNA revealed a single band of ~1200 nucleotides, comparable in size to the rat brain Yb3 mRNA. Divergence analysis of amino acid replacement sites in subunit 4 relative to the four rat Yb subunits revealed that it is most closely related to the brain-specific Yb3 subunit. This conclusion is further substantiated by the nucleotide sequence homology between {lambda}GTH4 and the Yb3 cDNA in their 3' untranslated region. In situ chromosome mapping has located this glutathione S-transferase gene in the region of p31 on chromosome 1. Results from many laboratories, including ours, indicate that the human glutathione S-transferases are encoded by a gene superfamily which is located on at least two different chromosomes.


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