Nucleic Acids Research, 1988, Vol. 16, No. 17 8571-8586
© 1988
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Conservation of histone H2A/H2B intergene regions: a role for the H2B specific element in divergent transcription
Department of Biochemistry, University of Adelaide PO Box 498, Adelaide, South Australia, 5000 Australia
Received January 6, 1988. Revised May 31, 1988. Accepted May 31, 1988.
The organization and function of potential regulatory elements associated with the promoters of chicken H2A and H2B genes pairs have been examined. The intergene regions of six dispersed and divergently-transcribed H2A/H2B gene pairs contain several extremely well conserved and spaced blocks of sequence homology. Adjacent coding regions are on average 342 base-pairs apart. Respective TATA boxes are separated by 180 base-pairs and within this confined region there are four CCAAT boxes and a previously identified 13 base-pair H2B-speclflc element (H2B-box) which has homology to the octamer motif present in a number of gene promoter/enhancer elements. Transcription of H2A and H2B genes from wild-type and mutant constructs was measured in transient assays by transfectlon into HeLa cells, and in permanently transformed clonal cell lines. In vitro separation of the two genes at a unique intergenlc site significantly decreased transcription of each gene. This suggested that the H2A/H2B gene pairs contained overlapping promoters. Deletion or point mutagenesls of the H2B-speclfic element decreased the levels of H2B and the H2A transcripts indicating that this sequence is a common regulatory element of both genes in the divergent-pair configeratlon.
*Present addresses: Cold Spring Harbor Laboratory, PO Box 100, Cold Spring Harbor, New York, NY 11724, USA
+Present addresses: Research Institute for Molecular Pathology, A-1030 Wien, Dr. Bohr-Gasse 7, Austria
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