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Nucleic Acids Research, 1988, Vol. 16, No. 17 8695-8705
© 1988

Articles

Structure of d(CACGTG), a Z-DNA hexamer containing AT base pairs

Miquel Coll, Ignacio Fita, Joaquim Lloveras, Juan A. Subirana*, Francese Bardella1, Tam Huynh-Dinh1 and Jean Igolen1

Unidad de Química Macromolecular, Escuela de Ingenieros Industriales Diagonal 647, 08028 Barcelona, Spain 1Unité de Chimie Organique, Départment de BGM, ERA-CNRS 927, Institut Pasteur 28 rue du Dr Roux, 75724 Paris Cedex 15, France

*To whom correspondence should be addressed

Received April 11, 1988. Revised July 6, 1988. Accepted July 6, 1988.

The left-handed Z-DNA conformation has been observed in crystals made from the self-complementary DNA hexamer d(CACGTG). This is the first time that a non disordered Z form is found in the crystal structure of an alternating sequence containing AT base pairs without methylated or brominated cytosines. The structure has been determined and refined to an agreement factor R=22.9% using 746 reflections in the resolution shell 7 to 2.5 a. The overall shape of the molecule is very similar to the Z-structure of the related hexamer d(CG)3 confirming the rigidity of the Z form. No solvent molecules were detected in the minor groove of the helix near the A bases. The disruption of the spine of hydration in the AT step appears to be a general fact in the Z form in contrast with the B form. The biological relevance of the structure in relation to the CA genome repeats is discussed.


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[Abstract] [PDF]


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