Nucleic Acids Research, 1988, Vol. 16, No. 18 8773-8787
© 1988
Articles |
Mouse DNA polymerase ß gene promoter: fine mapping and involvement of Sp1-like mouse transcription factor in its function
Laboratory of Cell Biology, Aichi Cancer Center Research Institute Chikusa-ku, Nagoya 464, Japan
*To whom correspondence should be addressed
Received July 15, 1988. Revised August 22, 1988. Accepted August 22, 1988.
The promoter of mouse DNA polymerase ß gene was analyzed by combining 5'-upstream region of this gene with chloramphenicol acetyltransferase (CAT) gene and by introduction of the recombinant plasmid DNA into mouse NIH/3T3 cells. Serial deletion of the mouse DNA sequence revealed that the promoter function resides within a 33 base pair region from the nucleotide position –48 to –15 with respect to the transcription initiation site, and is highly active without enhancer sequence. The promoter region was separated into two subregions: one (–48 to –35) contains a GC-box and the other contains a 10 base pair palindrome, whose sequence is similar to one of promoter consensus sequences found in a number of promoters including adenovirus promoters. The DNA polymerase ß promoter-directed CAT expression was competitively inhibited by the simultaneous transfection of plasmid DNA containing SV40 early promoter sequence. The viral sequences which are competitive to the GC-box of DNA polymerase ß gene promoter were the GC-boxes of SV40 promoter. Therefore, it is concluded that transcription of mouse DNA polymerase ß gene is regulated by mouse trans-acting factors equivalent to human Sp1 which is known to be trans-acting protein factor acting on SV40 GC-box sequences.
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