Skip Navigation

This Article
Right arrow Print PDF (728K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (14)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Terwilliger, T. C.
Right arrow Articles by Zabin, H. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Terwilliger, T. C.
Right arrow Articles by Zabin, H. B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 18 9027-9039
© 1988

Articles

A genetic selection for temperature-sensitive variants of the gene V protein of bacteriophage f1

Thomas C. Terwilliger*, Wilder D. Fulford1 and Hal B. Zabin

Department of Biochemistry and Molecular Biology, University of Chicago 920 E. 58th Street, Chicago, IL 60637 1Rockefeller University 1230 York Ave., New York, NY 10021, USA

*To whom correspondence should be addressed

Received April 1, 1988. Revised August 18, 1988. Accepted August 18, 1988.

Complementary negative and positive genetic selections based on the activity of a plasmid-encoded bacteriophage f1 gene V are developed. The negative selection is based on an activity of thegene V protein in E. coli cells which markedly reduces the infection of those cells by f1-related viruses. In order to select against cells expressing active gene V protein, the cells are infected with the phage R386, a derivative of f1 which confers resistance to chloramphenicol, and are plated in the presence of the antibiotic. Those cells which contain gene V protein are infrequently infected with the virus and are unable to grow in the presence of chloramphenicol; those which do not contain the gene V protein are readily infected and can grow in the presence of the antibiotic. The positive genetic selection consists of excising the gene V sequences from the plasmids and using them to replace the gene V of a bacteriophage f1 derivative containing an amber mutation in gene V. Only those genes which encode an active gene V protein can support phage growth and yield plaques. The two genetic selections can be combined in order to yield a substantial enrichment for genes encoding temperature-sensitive gene V proteins.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
J. L. Sachs and J. J. Bull
Experimental evolution of conflict mediation between genomes
PNAS, January 11, 2005; 102(2): 390 - 395.
[Abstract] [Full Text] [PDF]

Home page
 Protein Sci.Home page
T.-C. Mou, N. Sreerama, T. C. Terwilliger, R. W. Woody, and D. M. Gray
Independent tyrosyl contributions to the CD of Ff gene 5 protein and the distinctive effects of Y41H and Y41F mutants on protein-protein cooperative interactions
Protein Sci., March 1, 2002; 11(3): 601 - 613.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
W. Sandberg and T. Terwilliger
Influence of interior packing and hydrophobicity on the stability of a protein
Science, July 7, 1989; 245(4913): 54 - 57.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.