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Nucleic Acids Research, 1988, Vol. 16, No. 19 9147-9163
© 1988

Articles

RNA polymerase induces DNA bending at yeast mitochondria] promoters

Alfred H. Schinkel, Marian J.A. Groot Koerkamp, Aloys W.R.H. Teunissen and Henk F. Tabak*

Laboratory of Biochemistry, University of Amsterdam PO Box 20151, 1000 HD Amsterdam, The Netherlands

*To whom correspondence should be addressed

Received July 20, 1988. Accepted August 30, 1988.

Yeast mitochondrial RNA polymerase can bind specifically to promoter-containing DNA fragments in vitro as detected by DNAase I or methidiumpropyl-EDTA.Fe(II) protection assays and gel retardation experiments. Retardation of RNA polymerase-DNA complexes was most pronounced when the promoter was located in the middle of a DNA fragment and was diminished when RNA polymerase was bound near one of the ends. This indicates that upon RNA polymerase-binding the DNA undergoes a conformational change which is most likely a bend. The degree of introduced bending correlated with the efficiency of transcription and promoter-binding in a series of promoter mutants, suggesting that bending is a functional event during promoter utilisation.


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