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Nucleic Acids Research, 1988, Vol. 16, No. 2 455-470
© 1988

Articles

Molecular study of the retrovirus-like transposable element 412, a 20-OH ecdysone responsive repetitive sequence in Drosophila cultured cells

D. Micard, J.L. Couderc, M.L. Sobrier, G. Giraud and B. Dastugue

UA CNRS 360, Laboratorie de Biochimie Médicale, Faculté de Médecine BP 38, 28 Place H.Dunant, 63001 Clermont-Ferrand Cedex, France

Received October 22, 1987. Revised December 18, 1987. Accepted December 18, 1987.

Used at a physiological concentration, the steroid hormone 20-hydroxyecdysone (20-OHE) Induces, in Kc cultured Drosophlla melanogaster cells, important and specific changes. Modifications occur at morphological and enzymatical levels. Variations in specific protein synthesis are observed. At the molecular level, 20-OHE particularly Induces a decrease in expression of the mobile dispersed genetic element 412. This repeated element which belongs to the "copia-like" family is more widely represented In Kc cells (80 fold) compared to fly cells (25 fold). 412 transcripts are heterogeneous in size, essentially polyadenylated and restricted to the nucleus. A minimal concentration of 10–8 M and a time treatment of 16 hours are necessary to obtain a strong decrease in 412 expression. The decrease is at least an effect on these sequences at the transcriptlonal level. Structural similarities between the 412 element and the proviral forms of vertebrate retrovlruses are strenghtened by the characterization of extrachromosomal circular DNA forms revealed by the 412 probe. Quantifying experiments have shown that the steady state level of such forms is not affected by the steroid treatment.


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