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Nucleic Acids Research, 1988, Vol. 16, No. 2 471-486
© 1988

Articles

In vitro splicing of mRNA precursors: 5' cleavage site can be predicted from the interaction between the 5' splice region and the 5' terminus of U1 snRNA

Susanna Weber and Markus Aebi*

Institut fur Molekularbiologie I, Universität Zurich Hönggerberg, CH-8093 Zürich, Switzerland

*Present address: California Institute of Technology, Division of Biology 147-75, Pasadena, CA 91125, USA

Received October 22, 1987. Revised December 14, 1987. Accepted December 14, 1987.

Combinations of different mutations within the 5' splice region of the rabbit ß-glotrin large intron were analyzed for their effect on in vitro splicing. Based upon the complementarity of the 5' splice region to the 5' terminal region of the U1 snRNA, the exact location of the 5' cleavage site of different mutants could be predicted and was experimentally confirmed These findings add further strong support to the hypothesis (1) that the exact location of the 5' cleavage site in pre-mRNA splicing of higher eukaryotes is determined by the overall 5' splice region via the complementarity to the 5' end of the U1 snRNA, and not by the strongly conserved GU dinucleotide.


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