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Nucleic Acids Research, 1988, Vol. 16, No. 2 501-517
© 1988

Articles

Precise nucleosome positioning in the promoter of the chicken ßA globin gene

Panagiotis Kefalas, Fiona C. Gray and James Allan*

Department of Biophysics, Cell and Molecular Biology, King's, Queen's and Chelsea College 26–29 Drury Lane, London WC2B 5RL, UK

*To whom correspondence should be addressed

Received October 1, 1987. Revised December 16, 1987. Accepted December 16, 1987.

Histone octamers were reconstituted onto 5' endlabelled DNA fragments derived from the promoter region of the chicken ßA globin gene. The location of the reconstituted histone octamer with respect to the DNA sequence of each fragment was assessed by Exonuclease III digestion of purified nucleosome monomers. By this approach we have found a strong preference for histone octamers to be positioned over nucleotides –206 to –62 relative to the gene cap site. This stretch of DNA contains all those 5' ß globin sequences which, by DNase footprinting, bind specific protein factors (21,22) and incorporates three promoter concensus sequence motifs. The upstream terminal 32 base pairs of this DNA segment contains the binding sites for the erythrocyte specific G-string binding protein (21,22) and transcription factor Sp1 (23) and appears to be relatively weakly bound to the histone octamer.


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