Skip Navigation

This Article
Right arrow Print PDF (3123K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (54)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Faber, S. W.
Right arrow Articles by Wilcox, K. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Faber, S. W.
Right arrow Articles by Wilcox, K. W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 2 555-570
© 1988

Articles

Association of herpes simplex virus regulatory protein ICP4 with sequences spanning the ICP4 gene transcription initiation site

Steve W. Faber+ and Kent W. Wilcox*

Department of Microbiology, Medical College of Wisconsin Milwaukee, WI 53226, USA

+Present address: Department of Molecular Physiology & Biophysics, Vanderbilt School of Medicine, Nashville, TN 37232, U.S.A.

*To whom correspondence should be addressed

Received September 4, 1987. Revised November 10, 1987. Accepted November 10, 1987.

The HSV gene encoding ICP4 is negatively regulated and the HSV gene encoding thymidine kinase is positively regulated by ICP4 in vivo. We report that ICPA is a component of a stable complex that contains protein and a sequence of approximately 28 nucleotides that span the ICP4 gene transcription initiation site. The association of ICP4 with DNA sequences between positions –103 and +32 relative to the ICP4 mRNA start site was demonstrated by DNA binding immunoassays. DNase footprinting revealed that nucleotides between positions –8 and +20 are protected by ICP4. In contrast, binding of ICP4 to sequences flanking the mRNA start site in the thymidine kinase gene was not observed. Models for ICP4-mediated positive or negative regulation of HSV gene transcription are discussed.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
R. H. Kuddus and N. A. DeLuca
DNA-Dependent Oligomerization of Herpes Simplex Virus Type 1 Regulatory Protein ICP4
J. Virol., September 1, 2007; 81(17): 9230 - 9237.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. J. Advani, L. O. Durand, R. R. Weichselbaum, and B. Roizman
Oct-1 Is Posttranslationally Modified and Exhibits Reduced Capacity To Bind Cognate Sites at Late Times after Infection with Herpes Simplex Virus 1
J. Virol., November 15, 2003; 77(22): 11927 - 11932.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. W. Bruce and K. W. Wilcox
Identification of a Motif in the C Terminus of Herpes Simplex Virus Regulatory Protein ICP4 That Contributes to Activation of Transcription
J. Virol., January 1, 2002; 76(1): 195 - 207.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. Huang and L. A. Hanson
Temporal Gene Regulation of the Channel Catfish Virus (Ictalurid Herpesvirus 1)
J. Virol., March 1, 1998; 72(3): 1910 - 1917.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.