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Nucleic Acids Research, 1988, Vol. 16, No. 2 593-607
© 1988

Articles

RNA processing of beta-globin transcripts containing 5' flanking and structural gene sequences

Ilga Winicov

Departments of Biochemistry and Microbiology, University of Nevada Reno, NV 89557, USA

Received August 12, 1987. Revised November 30, 1987. Accepted November 30, 1987.

RNA species have been identified in murine erythroid cells which contain both 5' flanking and structural gene sequences from the betamaj globin gene. Two nonpolyadenylated RNA transcripts, average 3700 and 1800–1900 nucleotides long, were identified by denaturing agarose gel electrophoresis and were found to hybridize to both 5' and 3' betamaj globin flanking sequences. This finding suggests that transcription initiated in the 5' flanking region does proceed past the polyadenylation site. The apparent higher concentration of the 5' flanking sequences in precursor RNA molecules, as compared to mature polyadenylated globin mRNA, suggests increased precursor stability of beta globin transcripts initiated in the 5' flanking region.


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