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Nucleic Acids Research, 1988, Vol. 16, No. 20 9477-9487
© 1988

Articles

A site-specific single strand endonuclease activity induced by NYs-1 virus infection of a Chlorella-like green alga

Yuannan Xia+, Richard Morgan1, Ira Schildkraut1 and James L. Van Etten

Department of Plant Pathology, University of Nebraska Lincoln, NE 68583-0722 1New England Biolabs 32 Tozer Road, Beverly, MA 01915–9990, USA

Received August 7, 1988. Accepted September 20, 1988.

A site-specific endonuclease was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA-containing virus NYs-1. The enzyme recognizes the sequence 5'-CC-3' and cleaves 5' to the first C. It cleaves 5'-CmC-3' sequences but not 5'-mCC-3' sequences. The enzyme creates breaks in dsDNA whenever two 5'-CC-3' sequences on opposite strands are close enough for the two strands to separate; when the 5'-CC-3' sequences on opposite strands are further apart only a portion of the strands separate. Consequently, NYs-1 endonuclease does not produce a completely stable DNA digestion pattern. The enzyme probably does not cleave ssDNA and definitely does not cleave ssRNA or dsRNA.

+Present Address: Service d'Infectiologie, Centre Hospitalier de Université Laval, Ste-Foy, Quebec G1V 4G2, Canada


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