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Nucleic Acids Research, 1988, Vol. 16, No. 20 9497-9511
© 1988

Articles

Sigma region located between C, and C, genes of human immunoglobulin heavy chain: possible involvement of tRNA-like structure in RNA splicing

Yasushi Akahori, Hiroshi Handa1, Kenji Imai, Masumi Abe, Kohzoh Kameyama, Makoto Hibiya, Hisashi Yasui, Kazuhiko Okamura, Morihiro Naito, Hiroshi Matsuoka2 and Yoshikazu Kurosawa

nstitute for Comprehensive Medical Science, Fujita-Gakuen Health University Toyoake, Aichi 470-11 1Department of Bacteriology, Tokyo University School of Medicine Hongo, Bunkyo-ku, Tokyo 103 2Department of Pediatries, Nagoya University School of Medicine Tsurumai, Showa-ku, Nagoya 466, Japan

Received August 2, 1988. Accepted September 19, 1988.

Noncoding regions within the cluster of immunoglobulin heavy chain constant genes in the human genome contained a number of repeats. In the µ–{delta} intron, two repeating units were contained. One 442-base-long fragment located JH–µ intron ( defined as "sigma µ({sigma}µ)") occupied the position in the µ-{sigma} intron. The other 1166-base-long fragment located somewhere in front of S (class switch) region of C{gamma} gene was also found in the µ-{delta} intron. We defined the repeats in the µ-{delta} intron as "SIGMA ({Sigma})". The polarities of the longer repeats in the genome were opposite between the µ-{delta} intron and the upstreams of C{gamma} genes. These inverted copies (defined as {sigma}{gamma}3 and {sigma}{gamma}4), located 6 kb upstream of their respective C{gamma}'s, were apparently transcribed in vitro, via RNA polymerase III and transcripts should have contained tRNA-like structures. Small DNA fragments capable of encoding tRNA-like structures were also found in corresponding regions of mouse Ig C{gamma} cluster.

Present address: Department of Immunology, Chiba University, School of Medicine, Chiba, Japan (K.I. ).

Department of Immunology, Atomic Disease Institute, Nagasaki University School of Medicine, Sakamoto-cho, Nagasaki, Japan ( M.A. ).

Mitsubishi-Kasei Institute of Life Science, machida, Tokyo, Japan ( K.K ).


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