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Nucleic Acids Research, 1988, Vol. 16, No. 20 9545-9555
© 1988

Articles

A specific and efficient photoreaction between E.coli RNA polymerase and T+1 in the lacUV5 or deoPI promoter

Claus Jeppesen, Kaj Frank Jensen1 and Peter E. Nielsen*

Department of Biochemistry B, The Panum Institute, University of Copenhagen Blegdamsvej 3c, DK-2200 N Copenhagen 1Institute of Biological Chemistry B, University of Copenhagen Sølvgade 83, DK-1307 Copenhagen, Denmark

*To whom correspondence should be addressed

Received July 20, 1988. Accepted September 23, 1988.

Upon irradiation of the RNA polymerase-lacUV5 or deoPl promoter complex with short wavelength ultraviolet light ({lambda} ≤ 300 nm) the polymerase is covalently crosslinked at an efficiency of > 10% to the first transcribed base of the template DNA strand when this is a thymine. The temperature dependence of this RNA polymerase-T+1 photoreaction strongly indicates a relation to the formation of the open complex. It is suggested that open complex formation is preceeded or accompanied by a specific contact between the RNA polymerase and the first transcribed base of the DNA template.


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