Skip Navigation

This Article
Right arrow Print PDF (2712K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Philipsen, J. N. J.
Right arrow Articles by AB, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Philipsen, J. N. J.
Right arrow Articles by AB, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 20 9663-9676
© 1988

Articles

In vivo footprinting of the estrogen-inducible vitellogenin II gene from chicken

J. N. J. Philipsen, B. C. Hennis and G. AB

Department of Biochemistry, Groningen University Nijenborgh 16, 9747 AG Groningen, The Netherlands

Received July 15, 1988. Accepted September 16, 1988.

Protein-DNA interactions in the promoter region of the chicken vitellogenin II gene were analyzed by in vivo dimethyl sulphate footprinting with expressing and non-expressing tissues. The reactivity of G-residues is essentially the same in erythrocytes, oviduct and control liver, not expressing the gene. In the expressing estrogen-induced liver we find a number of G-residues with altered reactivities. These G's are located within distinct sequences: the estrogen responsive elements, a sequence resembling the NF-1 recognition motive, and several elements which are conserved between yolk protein genes. The expression-dependent binding of proteins to these sites was confirmed by DNasel footprinting applied to nuclei isolated from estrogen-induced and control liver. Estradiol appears to establish a transcription complex comprising a number of distinct proteins bound to different sites in the 5' flanking region of the vitellogenin II gene.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
D. Chen, P. E. Pace, R. C. Coombes, and S. Ali
Phosphorylation of Human Estrogen Receptor alpha  by Protein Kinase A Regulates Dimerization
Mol. Cell. Biol., February 1, 1999; 19(2): 1002 - 1015.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
R. S. Edinger, E. Mambo, and M. I. Evans
Estrogen-Dependent Transcriptional Activation and Vitellogenin Gene Memory
Mol. Endocrinol., December 1, 1997; 11(13): 1985 - 1993.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
F Weih, A F Stewart, M Boshart, D Nitsch, and G Schutz
In vivo monitoring of a cAMP-stimulated DNA-binding activity.
Genes & Dev., August 1, 1990; 4(8): 1437 - 1449.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.