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Nucleic Acids Research, 1988, Vol. 16, No. 21 10025-10036
© 1988

MOLECULAR BIOLOGY

A nicked group II intron and trans-splicing in liverwort, Marchantia polymorpha, chloroplasts

Takayuki Kohchi1,2, Kazuhiko Umesono3, Yutaka Ogura1, Yuriko Komine3, Kenji Nakahigashi3, Tohru Komano2, Yasuyuki Yamada1, Haruo Ozeki3 and Kanji Ohyama1,*

1Research Center for Cell and Tissue Culture, Faculty of Agriculture Kyoto University, Kyoto 606, Japan 2Department of Agricultural Chemistry, Faculty of Agriculture Kyoto University, Kyoto 606, Japan 3Department of Biophysics, Faculty of Science Kyoto University, Kyoto 606, Japan

* To whom correspondence should be addressed

Received August 16, 1988. Accepted September 27, 1988.

The chloroplast gene rps12 for ribosomal protein S12 in a liverwort, Marchantia polymorpha, is split into three exons by two introns, one of which (intron 1) is discontinuous. Exon 1 of rps12 for the N-terminal portion of the S12 protein is far from exons 2 and 3 for the C-terminal portion on the opposite DNA strand. S1-nuclease protection analysis and Northern hybridization with RNA isolated from the liverwort chloroplasts showed that: (i) the exons 1 and 2–3 of the rps12 gene with the neighboring genes were transcribed separately, (ii) the trans-splicing of intron 1 occurred after the processing of two primary transcripts to two pre-mRNAs, and (iii) there was no particular order for the splicing of intron 1 (trans) and intron 2 (cis) in the rps12 gene. We propose a bimolecular interaction model for trans-splicing by assuming that intermolecular base pairings between two pre-mRNAs result in the formation of the structure typical of group II introns except for disruption in the loop III region. This structure could be constructed in intron 1 of tobacco rps12 gene.


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