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Nucleic Acids Research, 1988, Vol. 16, No. 21 10225-10236
© 1988

MOLECULAR BIOLOGY

Bidirectional transfer from a 24 bp border repeat of Agrobacterium tumefaciens

M. J. J. van Haaren, N. J. A. Sedee, H. A. de Boer1, R. A. Schilperoort and P. J. J. Hooykaas

Molbas Research Group, Department of Plant Molecular Biology, Leiden University Wassenaarseweg 64, 2333 AL Leiden 1Department of Biochemistry, Gorlaeus Laboratory, Leiden University Wassenaarseweg 60, 2333 AL Leiden, The Netherlands

Received July 4, 1988. Accepted September 30, 1988.

T-region transfer from wild-type Agrobacterium strains is thought to be an orientated process, starting a t the right border repeat and terminating at the left border repeat of the T-region. Here we demonstrate that a right border repeat in the inverted orientation relative to the one-genes can also mediate transfer of the T-region to the plant cell, although with lower efficiency as a border repeat in the native orientation. Transfer mediated by an inverted right border repeat is stimulated by the presence of the T-region transfer enhancer. Similar single stranded molecules, comprising the bottom strand of the T-DNA, were isolated from acetosyringone induced bacteria, irrespective of the orientation of the right border. These findings show that border repeats work bidirectionally to some extent.

+Present address: Hahnemann University, Pathology Department, Broad and Vine, Philadelphia PA 19102-1192, USA


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