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Nucleic Acids Research, 1988, Vol. 16, No. 21 9933-9946
© 1988

MOLECULAR BIOLOGY

DNA supercolling and aerobic regulation of transcription from the Klebsiella pneumoniae nifLA promoter

R.A. Dixon, N. C. Hederson and S. Austin

AFRC Institute of Plant Science Research, Nitrogen Fixation Laboratory, University of Sussex Brighton, BN1 9RQ, UK

Received September 5, 1988. Accepted October 18, 1988.

Expression from the K. pneumoniae nifLA promoter is oxygen sensitive and is also inhibited by the DNA gyrase inhibitor coumermycin A1 under anaerobic growth conditions. The activity of this promoter was found to be highly sensitive to changes in DNA topology in vitro. Transcription was completely dependent on negative supercoiling at physiological salt concentrations although transcription from linear or fully relaxed closed circular templates was detectable at KC1 concentrations lower than 50 mM. These observations suggest that aerobic regulation of nif transcription may be mediated through the level of DNA supercoiling.


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