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Nucleic Acids Research, 1988, Vol. 16, No. 22 10413-10423
© 1988

MOLECULAR BIOLOGY

Impairment of yeast pre-mRNA splicing by potential secondary structure-forming sequences near the conserved branchpoint sequence

Hartmut Halfter and Dieter Gallwitz

Max-Planck-Institute for Biophysical Chemistry, Department of Molecular Genetics PO Box 2841, D-3400 Göttingen, FRG

Received October 5, 1988. Accepted October 24, 1988.

The absolutely conserved TACTAAC box within introns of RNA polymerase II-transcribed genes of the yeast Saccharomyces cerevisiae serves an indispensable role in lariat formation. We show in this report that rather short palindromic sequences inserted into the yeast actin gene intron immediately 3' to the TACTAAC box block the second but not the first splicing step. In contrast, a palindromic sequence inserted some 23 bp 3' of the TACTAAC box did not affect correct and efficient splicing. The data suggest that hairpin structures that might form adjacent to the branchsite sequence interfere with some necessary alteration of the spliceosome required for 3' intron cleavage and exon ligation.


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