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Nucleic Acids Research, 1988, Vol. 16, No. 22 10453-10468
© 1988

MOLECULAR BIOLOGY

Design, synthesis and expression of a human interteukin-2 gene incorporating the codon usage bias found in highly expressed Escherichia coli genes

D.P. Williams1, D. Regier, D. Akiyoshi, F. Genbauffe and J. R. Murphy

Evans Department of Clinical Research and Department of Medicine, The University Hospital 1Department of Microbiology, Boston University School of Medicine Boston, MA 02118, USA

Received September 15, 1988. Accepted October 18, 1988.

A synthetic gene encoding human interleulcin-2 (IL-2) was designed such that the codon usage bias resembled that found in highly expressed Escherichia coli genes. The percentage of preferred codons was increased from 431 in the native cDNA sequence to 85% in the synthetic sequence. The cDNA and synthetic IL-2 genes were placed under the control of the trc promoter and expressed in E. coli JM101. While Northern blot analysis of IL-2 mRNA from each genetic construct demonstrated equivalent message half-lives, immunoblot and bio-activity analyses showed the synthetic gene to direct the synthesis of up to 16 times more IL-2 than the native cDNA sequence.


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