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Nucleic Acids Research, 1988, Vol. 16, No. 22 10623-10634
© 1988

MOLECULAR BIOLOGY

Analysis of yeast chromosomal regions carrying members of the glutamate tRNA gene family: various transposable elements are associated with them

Joachim Hauber+, Rolf Stucka, Robert Krieg and Horst Feldmann*

Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität Schillerstraße 44, D-8000 München 2, FRG

*To whom correspondence should be addressed

Received July 26, 1988. Revised October 27, 1988. Accepted October 27, 1988.

We carried out an analysis on the genomic organisation of the tRNAGlu family in S.cerevisiae; eight clones were characterized by restriction mapping, hybridization and sequencing. These data taken together with our earlier findings show that the individual tRNAGlu 3 copies are identical only in their structural part but embedded in entirely different genomic environments. All of the tRNA genes identified here are flanked by elements such as Ty, delta, sigma, and tau. In some cases, sequences from different elements form complex patterns indicating a sophisticated history of these chromosomal regions. A novel observation is that Ty and delta in the regions analyzed are exclusively associated with the tRNA genes. The observed patterns imply that the tRNA genes mark regions of multiple transposition and subsequent excision events, but that these have occurred after the individual tRNA gene copies had been fixed in their present locations. Transcription experiments by the use of micro-injection into Xenopus oocytes suggest that the elements flanking the tRNA genes exert a modulating effect on their expression.

+Present address: The Howard Hughes Medical Institute, Duke University Medical Center, Box 3025, Durham, NC 27710, USA


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