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Nucleic Acids Research, 1988, Vol. 16, No. 22 10751-10764
© 1988

MOLECULAR BIOLOGY

Analysis of potential expression of highly related members of the ribosomal protein L32 gene family

Colleen M. Jacks1,+, Rebecca E. Biltz1 and Perry B. Hackett1,2

1Department of Genetics and Cell Biology, University of Minnesota 1445 Gortner Avenue, St Paul, MN 55108 2Institute of Human Genetics, University of Minnesota Minneapolis, MN 55455, USA

Received May 17, 1988. Revised October 17, 1988. Accepted October 24, 1988.

The processed gene L32', a member of the mouse gene family for ribosomal protein L32, could encode a 135 amino acid protein nearly identical to L32. The 5'-flanking region of the gene contains CAAT and TATA sites at positions commonly found in expressed genes. The L32' gene lies within highly methylated, DNase I-insensitive chromatin of mouse L1210 cells. Although S1 nuclease digestion studies suggested that an L32' transcript might be produced, an oligonucleotide probe specific for L32' mRNA, and RNase digestion of a cRNA probe to L32', indicated fewer than 0.1 L32' transcripts/cell. These results demonstrate that extreme caution is required when measuring transcription from related genes.

+Present address: Department of Molecular Genetics and Cell Biology, University of Chicago, 920 E. 58th St., Chicago, IL 60637, USA


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