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Nucleic Acids Research, 1988, Vol. 16, No. 22 10783-10802
© 1988

MOLECULAR BIOLOGY

Molecular cloning and nucleotide sequence of the nuclear PET122 gene required for expression of the mitochondrial COX3 gene in S.cerevisiae

Jeffrey D. Ohmen, Barbara Kloeckener-Gruissem1,+ and Joan E. McEwen*

Department of Microbiology and the Molecular Biology Institute, University of California at Los Angeles Los Angeles, CA 90024 1Department of Molecular, Celluar and Developmental Biology, University of Colorado Boulder, CO 80309, USA

*To whom correspondence should be addressed

Received April 29, 1988. Revised October 24, 1988. Accepted October 24, 1988.

The nuclear PET122 gene from S. cerevlslae is necessary for translation of a single mitochondrial mRNA that encodes subunit III of cytochrome c. oxidase. We report here the cloning and nucleotide sequence of PET122. and properties of the predicted protein product, which consists of 242 residues. Analysis of PET122-lacZ translational fusions confirms that the PET122 coding region is translated in. vivo and indicates that the PET122 protein product is targeted to mitochondria. A 117 residue domain located in the carboxy-terminal half of the PET122 protein, at least part of which is shown by characterization of mutants to be critical for PET122 function, exhibits 24% identity and 59% similarity to a portion of the catalytic domain of E. coli alanyl-tRNA synthetase. However, petl22 mutants are not defective in mitochondrial translation per se. as would be expected if PET122 encoded a tRNA synthetase. Instead, the PET122 protein may carry out one or more activities in common with tRNA synthetases, such as binding of ATP or RNA.

+Present address: Department of Genetics, University of California, Berkeley, CA 94720, USA


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